dianas 9(1) > García-Soriano etal

dianas | Vol 9 Num 1 | marzo 2020 | e202003d01

Evaluation of molecules with leishmanicidal activity in infected macrophages: SDS-LDH infection assay.

Juan Carlos García-Soriano^a, Héctor Elessar de Lucio-Ortega, Antonio Jiménez-Ruiz

Departamento de Biología de Sistemas, Universidad de Alcalá, Madrid, Spain.

a. jcarlos.garcias@uah.es; rosweil@hotmail.com

V Congreso de Señalización Celular, SECUAH 2020.
16-18 de marzo, 2020. Universidad de Alcalá. Alcalá de Henares, Madrid. España.

Keywords: Leishmania; infection assay; THP-1; SDS; LDH; flow cytometry

Abstract

Leishmaniasis is a neglected tropical disease that kills around 25,000 people a year worldwide for the most severe form of the disease, visceral leishmaniasis. This disease is caused by trypanosomatids of the Leishmania genus. The shortage of new drugs and the appearance of resistance makes it necessary to search for new compounds with leishmanicidal activity. Screening of compounds can be performed in free-living forms of the parasite or in macrophages infected with Leishmania, this option being the most relevant for the search for drug candidate compounds. This work shows the development of a new simple and fast method with high capacity that can be carried out using a flow cytometer, thus eliminating the need for confocal microscopy for high throughput screening technology.