{"id":79,"date":"2017-03-16T15:01:56","date_gmt":"2017-03-16T15:01:56","guid":{"rendered":"http:\/\/www3.uah.es\/biologiadesistemas\/secuah2017\/index.php\/secuah-2017-comunicacion-copy-6\/"},"modified":"2017-03-16T15:01:56","modified_gmt":"2017-03-16T15:01:56","slug":"dianas-2017-6-1-e20170305p03-ortiz-perez-y-sangari-characterization-of-omvs-from-brucella-abortus-2308","status":"publish","type":"post","link":"https:\/\/dianas.web.uah.es\/journal\/dianas-2017-6-1-e20170305p03-ortiz-perez-y-sangari-characterization-of-omvs-from-brucella-abortus-2308\/","title":{"rendered":"Dianas 6 (1) Ortiz-P\u00e9rez y Sangari 2017 Characterization of OMVs from Brucella abortus 2308."},"content":{"rendered":"<div class='ad_single_article'>\n<p class='ad_single_article_SO'><a href='https:\/\/dianas.web.uah.es\/journal\/dianas-6-1\/#dianas-2017-6-1-e20170305p03-ortiz-perez-y-sangari-characterization-of-omvs-from-brucella-abortus-2308'>Dianas 6(1)<br \/><\/a> &gt; <a href='https:\/\/dianas.web.uah.es\/journal\/dianas-2017-6-1-e20170305p03-ortiz-perez-y-sangari-characterization-of-omvs-from-brucella-abortus-2308\/'>Ortiz-P\u00e9rez y Sangari<br \/><\/a><\/p>\n\n<p class='signature'>Dianas | Vol 6 Num 1 | marzo  2017 | e20170305p03<br \/><\/p>\n\n<p class='w_title ad_single_article_title'>Characterization of OMVs from <i>Brucella abortus<\/i> 2308<br \/><\/p>\n\n<p class='w_authors ad_single_article_authors'>Yelina&nbsp;Ortiz&nbsp;P\u00e9rez<br&nbsp;\/>, F\u00e9lix&nbsp;J.&nbsp;Sangari<br&nbsp;\/><\/p>\n\n<p class='w_address ad_single_article_address'>Departamento de Biolog\u00eda Molecular, Universidad de Cantabria, IBBTEC, Santander (Cantabria).<br \/><\/p>\n\n<p class='w_mail ad_single_article_mail'>ortizy@unican.es, ortizy@unican.es<br \/><\/p>\n\n<p class='w_meeting ad_single_article_meeting'>II Congreso de Se\u00f1alizaci\u00f3n Celular, SECUAH 2017.<br \/><br \/>\n14-16 de marzo, 2017. Universidad de Alcal\u00e1. Alcal\u00e1 de Henares, Madrid. Espa\u00f1a.<br \/><br \/>\nSesi\u00f3n de paneles.<br \/><\/p>\n\n<p class='w_abstract_caption ad_single_article_abstract_caption'>Resumen<\/p>\n<p class='w_abstract ad_single_article_abstract'>Outer membrane vesicles (OMVs) were recently described as a new type of secretion system. Moreover, it has been observed that some bacteria can secrete two different types of OMVs, one formed only by outer membrane and periplasmic components, and a second type that includes also inner membrane and cytoplasmic components [1,2]. This opens the field to the presence in these vesicles of cytoplasmic components such as sRNAs. Among the multiple functions of these sRNAs, one of them is the interference with host functions, as it has been described for the first time for an <i>Escherichia coli<\/i> sRNA acting on <i>Caenorhabditis elegans<\/i> [3]. <i>Brucella<\/i> species produce such OMVs, and our hypothesis is that they probably contain both types of vesicles. If so, they could contain sRNAs with the potential of interfering with the host. To test this we have isolated and visualized <i>Brucella abortus<\/i> 2308 OMVs and their content by different techniques such as confocal microscopy, flow cytometry and electron microscopy, using <i>Shewanella vesiculosa<\/i> M7<sup>T<\/sup> to standardize all protocols. Our results indicate that the three methods are useful for different purposes, and thus complementary, although they need to be improved to fully demonstrate the presence or not of RNA in some of the OMVs. <\/p><\/p>\n\n<div class='ad_single_article_citation_box'>\n<\/div>\n\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,7,9,10,12,2],"tags":[],"class_list":["post-79","post","type-post","status-publish","format-standard","hentry","category-article","category-comunicacion","category-dianas-vol-6-num-1","category-journal","category-panel","category-vols-1-9"],"_links":{"self":[{"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/posts\/79","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/comments?post=79"}],"version-history":[{"count":0,"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/posts\/79\/revisions"}],"wp:attachment":[{"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/media?parent=79"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/categories?post=79"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dianas.web.uah.es\/journal\/wp-json\/wp\/v2\/tags?post=79"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}