dianas 7 (1) Matamoros-Recio etal 2018 Heteroaromatic azonia cations with application as DNA fluorescent probes.

dianas 7(1) > Matamoros-Recio etal

dianas | Vol 7 Num 1 | marzo 2018 | e201803p12

Heteroaromatic azonia cations with application as DNA fluorescent probes.

Universidad de Alcalá.

aalejandra-m-r@hotmail.es

III Congreso de Señalización Celular, SECUAH 2018.
20-22 de marzo, 2018. Universidad de Alcalá. Alcalá de Henares, Madrid. España.
Sesión de paneles

Resumen

The fluorimetric detection and visualization of biomacromolecules is a key technique in bioanalytical chemistry. Fluorescent probes whose emission intensity increases upon association with biomacromolecules such as DNA or proteins (“light-up probes”) are useful markers in genomics and proteomics. These probes can be used to stain subcellular structures in fluorescence microscopy, flow citometry and cell sorting, or to stain nucleic acids and proteins in gel electrophoresis. Condensed polyaromatic azonia cations are a family with good fluorescent and DNA intercalating properties. However, the fluorescence intensity of most of these tested cations is efficiently quenched upon complexation with DNA. Herein, we report a small library of azonia cations based on benzimidazolium core. Some of them have shown a significantly fluorescence increase upon DNA addition and promising properties as live-cell fluorescent stains in confocal microscopy of live HeLa cells.

Cita: Matamoros Recio, Alejandra; Bosch, Pedro; Sucunza, David; Mendicuti, Francisco; Vaquero, Juan José; Domingo, Alberto (2018) Heteroaromatic azonia cations with application as DNA fluorescent probes. Actas del III Congreso de Señalización Celular, SECUAH 2018. 20-22 de marzo, 2018. Universidad de Alcalá. Alcalá de Henares, Madrid. España. Sesión de paneles. dianas 7 (1): e201803p12. ISSN 1886-8746 (electronic) journal.dianas.e201803p12 http://www3.uah.es/dianas?e201803p12. URI http://hdl.handle.net/10017/15181

Copyright: © Matamoros-Recio A, Bosch P, Sucunza D, Mendicuti F, Vaquero JJ, Domingo A. Algunos derechos reservados. Este es un artículo open-access distribuido bajo los términos de una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. http://creativecommons.org/licenses/by-nc-nd/4.0/

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