dianas 10 (1) Moreno-Gómez‑Toledano etal 2021 Bisphenol A impaired cell adhesion by altering the expression of adhesion and cytoskeleton proteins on human podocytes.

dianas 10(1) > Moreno-Gómez‑Toledano etal

dianas | Vol 10 Num 1 | marzo 2021 | e202103b01

Bisphenol A impaired cell adhesion by altering the expression of adhesion and cytoskeleton proteins on human podocytes.

Universidad de Alcalá (UAH).

ar.morenogomeztoledano@gmail.com; rafael.moreno@uah.es

VI Congreso de Señalización Celular, SECUAH 2021.
29 de marzo a 30 de abril, 2021. Universidad de Alcalá. Alcalá de Henares, Madrid. España.

Abstract

Bisphenol A (BPA), a chemical -xenoestrogen- used in food containers is present in the urine of almost the entire population. Recently, several extensive population studies have proven a significant association between urinary excretion of BPA and albuminuria. The alteration of glomerular podocytes or "podocytopathy" is a common event in chronic albuminuric conditions. Since many podocytes recovered from patients' urine are viable, we hypothesized that BPA could impair podocyte adhesion capabilities. Using an in vitro adhesion assay, we observed that BPA impaired podocyte adhesion, an effect that was abrogated by Tamoxifen (an estrogen receptor blocker). Genomic and proteomic analyses revealed that BPA affected the expression of several podocyte cytoskeleton and adhesion proteins. Western blot and immunocytochemistry confirmed the alteration in the protein expression of tubulin, vimentin, podocin, cofilin-1, vinculin, E-cadherin, nephrin, VCAM-1, tenascin-C, and β-catenin. Moreover, we also found that BPA, while decreased podocyte nitric oxide production, it lead to overproduction of ion superoxide. In conclusion, our data show that BPA induced a novel type of podocytopathy characterizes by an impairment of podocyte adhesion, by altering the expression of adhesion and cytoskeleton proteins. Moreover, BPA diminished production of podocyte nitric oxide and induced the overproduction of oxygen-free metabolites. These data provide a mechanism by which BPA could participate in the pathogenesis and progression of renal diseases.

Citation: Moreno Gómez‑Toledano, Rafael; Arenas, María I.; González‑Martínez, Clara; Olea‑Herrero, Nuria; Reventún, Paula; Di Nunzio, Michele; Sánchez‑Esteban, Sandra; Arilla‑Ferreiro, Eduardo; Saura, Marta; Bosch, Ricardo J. (2021) Bisphenol A impaired cell adhesion by altering the expression of adhesion and cytoskeleton proteins on human podocytes. Proceedings of the VI Congreso de Señalización Celular, SECUAH 2021. 29 de marzo a 30 de abril, 2021. Universidad de Alcalá. Alcalá de Henares, Madrid. España. dianas 10 (1): e202103b01. ISSN 1886-8746 (electronic) journal.dianas.e202103b01 http://www3.uah.es/dianas?e202103b01. URI http://hdl.handle.net/10017/15181

Copyright: © Moreno-Gómez‑Toledano R, Arenas MI, González‑Martínez C, Olea‑Herrero N, Reventún P, Di-Nunzio M, Sánchez‑Esteban S, Arilla‑Ferreiro E, Saura M, Bosch RJ. Some rights reserved. This is an open-access work licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. http://creativecommons.org/licenses/by-nc-nd/4.0/

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