Dianas 2(1) > Bejarano-Prudencio
Dianas | Vol 2 Num 1 | marzo 2013 | e20130302
Cell-Based High-Throughput Screening Assay for Identification of G-Protein-Coupled Receptors Agonists and Antagonists.
GlaxoSmithKline R&D Pharmaceuticals, Screening and Compound Profiling. Centro de Investigación Básica (C.I.B.) Santiago Grisolía, 4. 28760 Tres Cantos (Madrid).
albertobejarano@hotmail.com
Palabras clave: Ca2+ mobilization; GPCRs; FLIPR; fluorescent Ca2+ dye; high-throughput screening
Resumen
The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobilization. The emergence of the fluorescent imaging plate reader and Ca2+-sensitive fluorescent dyes has made the high-content screening of GPCR possible. Here was applied a dual automated FLIPR platform to testing a large collection of compounds to identification of both agonist and antagonists form a single screen of GPCR.
Cita: Bejarano Prudencio, Alberto (2013) Cell-Based High-Throughput Screening Assay for Identification of G-Protein-Coupled Receptors Agonists and Antagonists. Dianas 2 (1): e20130302. ISSN 1886-8746 (electronic) journal.dianas.e20130302 https://dianas.web.uah.es/journal/e20130302. URI http://hdl.handle.net/10017/15181
Copyright: © Bejarano-Prudencio A. Algunos derechos reservados. Este es un artículo open-access distribuido bajo los términos de una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional. http://creativecommons.org/licenses/by-nc-nd/4.0/
